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The 2 protein bands from 40 kDa to 30 kDa ended up absent soon after 24 h incubation at 45 °C. For the rest of the bands, many concentrations of all three proteases retained protein bands comparable to their unique molar masses (Figure 6a,b). The existence of bands at minimal molecular excess weight in fish roe ahead of hydrolysis was surprising. During the report by Ghelichi et al. [8], slight peaks of a comparatively minimal molecular pounds assortment (three–14 kDa) were being observed in defatted carp roe homogenate, most likely as a result of an autolytic system occurring in fish roe and/or perhaps the defatting procedure resulting in the formation of very low-molecular-weight peptides.

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